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Addgene

pFLAG-SURF4
(Plasmid #170680)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 170680 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAc-GFPC1-Sec61beta
  • Backbone manufacturer
    Tom Rapoport Lab (Addgene #15108)
  • Backbone size w/o insert (bp) 3978
  • Total vector size (bp) 4903
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SURF4
  • Species
    Chlorocebus sabaeus
  • Insert Size (bp)
    925
  • GenBank ID
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV Forward
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFLAG-SURF4 was a gift from Ke Xu (Addgene plasmid # 170680 ; http://n2t.net/addgene:170680 ; RRID:Addgene_170680)

  • For your References section:

    SURF4-induced tubular ERGIC selectively expedites ER-to-Golgi transport. Yan R, Chen K, Wang B, Xu K. Dev Cell. 2022 Feb 28;57(4):512-525.e8. doi: 10.1016/j.devcel.2021.12.018. Epub 2022 Jan 19. 10.1016/j.devcel.2021.12.018 PubMed 35051356
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