pStr-KDEL_APV-SBP-Dendra2-ER-5
(Plasmid
#170679)
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PurposeExpresses ER-targeted streptavidin-KDEL (hook) and SBP-Dendra2 targeted to the ER lumen with an N-terminal APV tripeptide (cargo) for RUSH.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 170679 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneStr-KDEL_SBP-EGFP-Ecadherin
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Backbone manufacturerFranck Perez Lab (Addgene #65286)
- Backbone size w/o insert (bp) 4407
- Total vector size (bp) 5922
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameAPV-SBP-Dendra2-ER-5
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SpeciesSynthetic
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Insert Size (bp)903
- Promoter IRES
Cloning Information for Gene/Insert 1
- Cloning method Gibson Cloning
- 5′ sequencing primer IRES-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert namestreptavidin-KDEL
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SpeciesSynthetic
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Insert Size (bp)624
- Promoter T7
Cloning Information for Gene/Insert 2
- Cloning method Unknown
- 5′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pStr-KDEL_APV-SBP-Dendra2-ER-5 was a gift from Ke Xu (Addgene plasmid # 170679 ; http://n2t.net/addgene:170679 ; RRID:Addgene_170679) -
For your References section:
SURF4-induced tubular ERGIC selectively expedites ER-to-Golgi transport. Yan R, Chen K, Wang B, Xu K. Dev Cell. 2022 Feb 28;57(4):512-525.e8. doi: 10.1016/j.devcel.2021.12.018. Epub 2022 Jan 19. 10.1016/j.devcel.2021.12.018 PubMed 35051356