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pStr-KDEL_EPV-CXCL9-mCherry-SBP
(Plasmid #170678)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 170678 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Str-KDEL_SBP-EGFP-Ecadherin
  • Backbone manufacturer
    Franck Perez Lab (Addgene #65286)
  • Backbone size w/o insert (bp) 4407
  • Total vector size (bp) 6277
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    CXCL9
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    1246
  • Mutation
    Changed threonine 23 to glutamate (T23E)
  • GenBank ID
    NM_002416.2
  • Entrez Gene
    CXCL9 (a.k.a. CMK, Humig, MIG, SCYB9, crg-10)
  • Promoter IRES
  • Tags / Fusion Proteins
    • mCherry (C terminal on insert)
    • SBP (C terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site AscI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer IRES-F
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    streptavidin-KDEL
  • Species
    Synthetic
  • Insert Size (bp)
    624
  • Promoter T7

Cloning Information for Gene/Insert 2

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pStr-KDEL_EPV-CXCL9-mCherry-SBP was a gift from Ke Xu (Addgene plasmid # 170678 ; http://n2t.net/addgene:170678 ; RRID:Addgene_170678)

  • For your References section:

    SURF4-induced tubular ERGIC selectively expedites ER-to-Golgi transport. Yan R, Chen K, Wang B, Xu K. Dev Cell. 2022 Feb 28;57(4):512-525.e8. doi: 10.1016/j.devcel.2021.12.018. Epub 2022 Jan 19. 10.1016/j.devcel.2021.12.018 PubMed 35051356
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