Skip to main content
Addgene

pRha-ABE7.10-SpCas9-NG
(Plasmid #170663)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 170663 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pD881-SR
  • Backbone manufacturer
    Atum
  • Backbone size w/o insert (bp) 2256
  • Total vector size (bp) 7707
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    ABE7.10-SpCas9-NG
  • Species
    Synthetic
  • Insert Size (bp)
    5451
  • Promoter pRhaBAD
  • Tags / Fusion Proteins
    • 8xHIS (N terminal on insert)
    • BP-NLS (N terminal on insert)
    • BP-NLS (C terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer CGAATTCAGGCGCTTTTTAG
  • 3′ sequencing primer CAGTGAGTTGATTGCAGTCC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRha-ABE7.10-SpCas9-NG was a gift from David Liu (Addgene plasmid # 170663 ; http://n2t.net/addgene:170663 ; RRID:Addgene_170663)

  • For your References section:

    Functional correction of CFTR mutations in human airway epithelial cells using adenine base editors. Krishnamurthy S, Traore S, Cooney AL, Brommel CM, Kulhankova K, Sinn PL, Newby GA, Liu DR, McCray PB. Nucleic Acids Res. 2021 Oct 11;49(18):10558-10572. doi: 10.1093/nar/gkab788. 10.1093/nar/gkab788 PubMed 34520545
Related items:
Commonly requested with: