-
PurposeLentiviral expression of constitutively active STAT5A.
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 170662 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepTRIPZ
-
Vector typeLentiviral
-
Selectable markersPuromycin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameSTAT5A
-
SpeciesH. sapiens (human)
-
MutationH298R and S710F
-
Entrez GeneSTAT5A (a.k.a. MGF, STAT5)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
A constitutively active form of STAT5A was cloned into the lentiviral expression vector.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pBR-Lent-STAT5A-CA was a gift from Alexander Ruthenburg (Addgene plasmid # 170662 ; http://n2t.net/addgene:170662 ; RRID:Addgene_170662) -
For your References section:
Non-canonical H3K79me2-dependent pathways promote the survival of MLL-rearranged leukemia. Richter WF, Shah RN, Ruthenburg AJ. Elife. 2021 Jul 15;10. pii: 64960. doi: 10.7554/eLife.64960. 10.7554/eLife.64960 PubMed 34263728
