pGEX Syt1 C2AB (96-421) I367T F234C
(Plasmid
#170653)
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PurposeExpression of syt1 for purification in E. Coli
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 170653 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGEX-4T
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameSYT1
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SpeciesR. norvegicus (rat)
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MutationI367T, C277A, F234C
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Entrez GeneSyt1 (a.k.a. P65)
Cloning Information
- Cloning method Ligation Independent Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEX Syt1 C2AB (96-421) I367T F234C was a gift from Edwin Chapman (Addgene plasmid # 170653 ; http://n2t.net/addgene:170653 ; RRID:Addgene_170653) -
For your References section:
Molecular Basis for Synaptotagmin-1-Associated Neurodevelopmental Disorder. Bradberry MM, Courtney NA, Dominguez MJ, Lofquist SM, Knox AT, Sutton RB, Chapman ER. Neuron. 2020 Jul 8;107(1):52-64.e7. doi: 10.1016/j.neuron.2020.04.003. Epub 2020 May 1. 10.1016/j.neuron.2020.04.003 PubMed 32362337