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Addgene

pGEX Syt1 C2AB (96-421) D303G V304C
(Plasmid #170648)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 170648 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-4T
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    SYT1
  • Species
    R. norvegicus (rat)
  • Mutation
    D303G, C277A, V304C
  • Entrez Gene
    Syt1 (a.k.a. P65)

Cloning Information

  • Cloning method Ligation Independent Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX Syt1 C2AB (96-421) D303G V304C was a gift from Edwin Chapman (Addgene plasmid # 170648 ; http://n2t.net/addgene:170648 ; RRID:Addgene_170648)

  • For your References section:

    Molecular Basis for Synaptotagmin-1-Associated Neurodevelopmental Disorder. Bradberry MM, Courtney NA, Dominguez MJ, Lofquist SM, Knox AT, Sutton RB, Chapman ER. Neuron. 2020 Jul 8;107(1):52-64.e7. doi: 10.1016/j.neuron.2020.04.003. Epub 2020 May 1. 10.1016/j.neuron.2020.04.003 PubMed 32362337
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