ZE22 vox-CS (ZV100)
(Plasmid
#17064)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 17064 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCS2+
- Backbone size w/o insert (bp) 4100
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Vector typeZebrafish Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namevox-2
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Alt nameZvox-2
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Alt namevox
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SpeciesD. rerio (zebrafish)
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Insert Size (bp)750
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Entrez Genevox (a.k.a. vega1, zgc:136532)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Zvox-2 coding region amplified by PCR and inserted into CS2+ (first cloned into SK as zV93).
For RNA: cut with Asp718 and transcribe with SP6.
Plasmid History:
Species of Sequence Origin: Zebrafish.
Constructed by: David Kimelman, 6/28/97 XIX p. 67.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
ZE22 vox-CS (ZV100) was a gift from Randall Moon (Addgene plasmid # 17064 ; http://n2t.net/addgene:17064 ; RRID:Addgene_17064)
Map uploaded by the depositor.