pLIC-lyn11-myc-LOV2GIVe (FA)
(Plasmid
#170626)
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PurposeFor the mammalian expression of mLOV2GIVe GEF-deficient mutation F1685A
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 170626 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLIC-myc
- Backbone size w/o insert (bp) 6000
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLyn11-myc-LOV2GIVe (FA)
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SpeciesSynthetic
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Insert Size (bp)594
- Promoter CMV
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Tag
/ Fusion Protein
- myc (N terminal on backbone)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLIC-lyn11-myc-LOV2GIVe (FA) was a gift from Mikel Garcia-Marcos (Addgene plasmid # 170626 ; http://n2t.net/addgene:170626 ; RRID:Addgene_170626) -
For your References section:
Optogenetic activation of heterotrimeric G-proteins by LOV2GIVe, a rationally engineered modular protein. Garcia-Marcos M, Parag-Sharma K, Marivin A, Maziarz M, Luebbers A, Nguyen LT. Elife. 2020 Sep 16;9. pii: 60155. doi: 10.7554/eLife.60155. 10.7554/eLife.60155 PubMed 32936073
