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Addgene

pLIC-lyn11-myc-LOV2GIVe (L)
(Plasmid #170624)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 170624 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pLIC-myc
  • Backbone size w/o insert (bp) 6000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Lyn11-myc-LOV2GIVe (L)
  • Species
    Synthetic
  • Insert Size (bp)
    594
  • Promoter CMV
  • Tag / Fusion Protein
    • myc (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLIC-lyn11-myc-LOV2GIVe (L) was a gift from Mikel Garcia-Marcos (Addgene plasmid # 170624 ; http://n2t.net/addgene:170624 ; RRID:Addgene_170624)
  • For your References section:

    Optogenetic activation of heterotrimeric G-proteins by LOV2GIVe, a rationally engineered modular protein. Garcia-Marcos M, Parag-Sharma K, Marivin A, Maziarz M, Luebbers A, Nguyen LT. Elife. 2020 Sep 16;9. pii: 60155. doi: 10.7554/eLife.60155. 10.7554/eLife.60155 PubMed 32936073