pAPV-DsRed2-FLAG-ER-5
(Plasmid
#170622)
-
PurposeExpresses DsRed2-FLAG targeted to the ER lumen with an N-terminal APV tripeptide after signal peptide cleavage. The protein is efficiently retrieved due to exposure of KDEL.
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 170622 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepDendra2-C
-
Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4026
- Total vector size (bp) 4767
-
Vector typeMammalian Expression
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameAPV-DsRed2-ER-5
-
SpeciesSynthetic
-
Insert Size (bp)741
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BmtI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer CMV Forward
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pAPV-DsRed2-FLAG-ER-5 was a gift from Ke Xu (Addgene plasmid # 170622 ; http://n2t.net/addgene:170622 ; RRID:Addgene_170622) -
For your References section:
SURF4-induced tubular ERGIC selectively expedites ER-to-Golgi transport. Yan R, Chen K, Wang B, Xu K. Dev Cell. 2022 Feb 28;57(4):512-525.e8. doi: 10.1016/j.devcel.2021.12.018. Epub 2022 Jan 19. 10.1016/j.devcel.2021.12.018 PubMed 35051356