ZE9 tiggywinkle hedgehog pT7TS
(Plasmid #17058)

• Depositing Lab: Randall Moon
• Publication: Ekker et al Curr Biol. 1995 Aug 1. 5(8):944-55.

Backbone

• Vector backbone: T7TS
• Backbone size w/o insert (bp): 3000
• Vector type: Zebrafish Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: tiggywinkle hedgehog
• Alt name: twhh
• Species: D. rerio (zebrafish)
• Entrez Gene: shhb (a.k.a. twh, twhh)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SpeI (unknown if destroyed)
• 3′ cloning site: SpeI (unknown if destroyed)
• 5′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Steve Ekker clone A, in vector pT7TS.
Citation: Ekker et al. (1995), Current Biology 5:944-955.

T7TS-twhh was made by insertion of twhh open-reading frame into the SpeI site of the pT7TS vector. This vector adds Xenopus laevis β-globin 5′ and 3′ untranslated sequences to the inserted open-reading frame for increased transcript stability. The ORF was made by PCR using the following primers and appropriate cDNA as template:(5′-GGACTAGTCACCATGGA-CGTAAGGCTGCA-3′), and (5′-AGCATACTAGTA-GTCTCAACTTAAGTGTAA-3′).