ATGL*-V5-APEX2
(Plasmid #170574)

• Purpose: A C-terminal APEX2 fusion to the human ATGL S47A protein
• Depositing Lab: James Olzmann
• Publication: Bersuker et al Dev Cell. 2018 Jan 8;44(1):97-112.e7. doi: 10.1016/j.devcel.2017.11.020. Epub 2017 Dec 21.

Backbone

• Vector backbone: pLenti CMV/TO Puro DEST
• Backbone size w/o insert (bp): 9594
• Total vector size (bp): 11901
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: ATGL*
• Species: H. sapiens (human)
• Insert Size (bp): 2307
• Mutation: changed serine 47 to alanine
• Entrez Gene: PNPLA2 (a.k.a. 1110001C14Rik, ATGL, FP17548, PEDF-R, TTS-2.2, TTS2, iPLA2zeta)
• Promoter: CMV
• Tag / Fusion Protein:
  • V5-APEX2 (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV forward
• 3′ sequencing primer: WPRE-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry