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Addgene

PLIN2-V5-APEX2
(Plasmid #170573)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 170573 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLenti CMV/TO Puro DEST
  • Backbone size w/o insert (bp) 9594
  • Total vector size (bp) 11640
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PLIN2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2034
  • Entrez Gene
    Plin2 (a.k.a. ADPH, Adfp, Adrp)
  • Promoter CMV
  • Tag / Fusion Protein
    • V5-APEX2 (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PLIN2-V5-APEX2 was a gift from James Olzmann (Addgene plasmid # 170573 ; http://n2t.net/addgene:170573 ; RRID:Addgene_170573)

  • For your References section:

    A Proximity Labeling Strategy Provides Insights into the Composition and Dynamics of Lipid Droplet Proteomes. Bersuker K, Peterson CWH, To M, Sahl SJ, Savikhin V, Grossman EA, Nomura DK, Olzmann JA. Dev Cell. 2018 Jan 8;44(1):97-112.e7. doi: 10.1016/j.devcel.2017.11.020. Epub 2017 Dec 21. 10.1016/j.devcel.2017.11.020 PubMed 29275994
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