Skip to main content
Addgene

ZE7 Pax2
(Plasmid #17056)

Print

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17056 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    SP64T
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Zebrafish Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    pax b
  • Alt name
    pax2
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    1180
  • Entrez Gene
    pax2a (a.k.a. PAXZF-B, Pax-2, cb378, noi, pax-b, pax2.1, pax2a1, pax[zf-b], paxb)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bgl II (unknown if destroyed)
  • 3′ cloning site BglII (unknown if destroyed)
  • 5′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Insert: pax b sense or antisense orientation.
Insert size: 1.18 Kb.
Transcription: linearize at EcoR1, transcribe with SP6.

Plasmid History:
Constructed by Greg Kelly on date of 9/21/92, page 118 Book 1.
Sepecies of Sequence Origin: Zebrafish.

Note for Use:
This construct is used to make DIG probes or for making RNA suitable for injections.
Citation: Kelly and Moon, Developmental Genetics (1995)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ZE7 Pax2 was a gift from Randall Moon (Addgene plasmid # 17056 ; http://n2t.net/addgene:17056 ; RRID:Addgene_17056)

  • For your References section:

    Involvement of wnt1 and pax2 in the formation of the midbrain-hindbrain boundary in the zebrafish gastrula. Kelly GM, Moon RT. Dev Genet. 1995. 17(2):129-40. 10.1002/dvg.1020170205 PubMed 7586754
Related items:
Commonly requested with: