pFastBac Dual α1B/β2B Tubulin
(Plasmid #170553)

• Purpose: Encodes codon-optimized human α1B and β2B tubulin for expression in and purification from insect cells.
• Depositing Lab: Tarun Kapoor
• Publication: Ti et al Dev Cell. 2018 Oct 22;47(2):175-190.e5. doi: 10.1016/j.devcel.2018.08.014. Epub 2018 Sep 20.

Backbone

• Vector backbone: pFastBac Dual
• Backbone manufacturer: Thermo Fisher
• Backbone size w/o insert (bp): 5238
• Total vector size (bp): 8026
• Vector type: Insect Expression
• Selectable markers: Gentamicin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: TUBA1B
• Species: H. sapiens (human)
• GenBank ID: NP_006073.2
• Entrez Gene: TUBA1B (a.k.a. K-ALPHA-1)
• Tags / Fusion Proteins:
  • L21 enhancer (N terminal on insert)
  • His10 tag (N terminal on insert)
  • TEV site (N terminal on insert)
  • AP linker (N terminal on insert)

Gene/Insert 2

• Gene/Insert name: TUBB2B
• Species: H. sapiens (human)
• GenBank ID: NM_178012.4
• Entrez Gene: TUBB2B (a.k.a. PMGYSA, bA506K6.1)
• Tags / Fusion Proteins:
  • L21 enhancer (N terminal on insert)
  • TEV site (C terminal on insert)
  • GS linker (GGSGG) (C terminal on insert)
  • StrepTagII (C terminal on insert)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

See Ti et al. STAR Protocol from 2020 (citation below) for details on expression and purification.

Ti SC, Wieczorek M, Kapoor TM. Purification of Affinity Tag-free Recombinant Tubulin from Insect Cells. STAR Protoc. 2020 Jun 19;1(1):100011. doi: 10.1016/j.xpro.2019.100011. Epub 2020 Jun 3. PMID: 32783031; PMCID: PMC7416841.