mCherry-GFP-SNX9
(Plasmid
#170551)
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PurposeTo monitor the turnover of SNX9 in lysosomes
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 170551 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBabe
- Total vector size (bp) 8364
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Vector typeMammalian Expression, Retroviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSNX9
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SpeciesH. sapiens (human)
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Insert Size (bp)1790
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GenBank IDNM_016224.5
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Entrez GeneSNX9 (a.k.a. SDP1, SH3PX1, SH3PXD3A, WISP)
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Tag
/ Fusion Protein
- SNX9 in tandem with mCherry and GFP
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site SalI (unknown if destroyed)
- 5′ sequencing primer eGFP
- 3′ sequencing primer SV40 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mCherry-GFP-SNX9 was a gift from Christina Towers (Addgene plasmid # 170551 ; http://n2t.net/addgene:170551 ; RRID:Addgene_170551) -
For your References section:
Mitochondrial-derived vesicles compensate for loss of LC3-mediated mitophagy. Towers CG, Wodetzki DK, Thorburn J, Smith KR, Caino MC, Thorburn A. Dev Cell. 2021 Jul 26;56(14):2029-2042.e5. doi: 10.1016/j.devcel.2021.06.003. Epub 2021 Jun 24. 10.1016/j.devcel.2021.06.003 PubMed 34171288