pME-MmCav1
(Plasmid
#170527)
-
PurposeEntry clone for tagging MmCav1 at the C terminus. Parton lab clone BCJ
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 170527 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepDONR 221
-
Vector typemultisite gateway entry clone
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pME-MmCav1 was a gift from Rob Parton (Addgene plasmid # 170527 ; http://n2t.net/addgene:170527 ; RRID:Addgene_170527) -
For your References section:
A robust method for particulate detection of a genetic tag for 3D electron microscopy. Rae J, Ferguson C, Ariotti N, Webb RI, Cheng HH, Mead JL, Riches JD, Hunter DJ, Martel N, Baltos J, Christopoulos A, Bryce NS, Cagigas ML, Fonseka S, Sayre ME, Hardeman EC, Gunning PW, Gambin Y, Hall TE, Parton RG. Elife. 2021 Apr 27;10. pii: 64630. doi: 10.7554/eLife.64630. 10.7554/eLife.64630 PubMed 33904409