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Addgene

LifeAct-EGFP-APEX2 in pCSDEST2
(Plasmid #170523)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 170523 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCSDEST2 (Addgene 22424)
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cavin4
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Cavin4 (a.k.a. 2310039E09Rik, Mur, Murc)
  • Tag / Fusion Protein
    • APEX2-P2A-mKate2 (C terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LifeAct-EGFP-APEX2 in pCSDEST2 was a gift from Rob Parton (Addgene plasmid # 170523 ; http://n2t.net/addgene:170523 ; RRID:Addgene_170523)

  • For your References section:

    A robust method for particulate detection of a genetic tag for 3D electron microscopy. Rae J, Ferguson C, Ariotti N, Webb RI, Cheng HH, Mead JL, Riches JD, Hunter DJ, Martel N, Baltos J, Christopoulos A, Bryce NS, Cagigas ML, Fonseka S, Sayre ME, Hardeman EC, Gunning PW, Gambin Y, Hall TE, Parton RG. Elife. 2021 Apr 27;10. pii: 64630. doi: 10.7554/eLife.64630. 10.7554/eLife.64630 PubMed 33904409
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