pXL-BacII-CAG-loxP-STOP-loxP-Venus
(Plasmid
#170520)
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PurposePlasmid containing piggyBac ITRs flanking CAG, a loxP-STOP-loxP cassette and Venus (also see doi: 10.7554/eLife.63907 )
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 170520 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepXL-BacII
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCAG-loxP-STOP-loxP-Venus
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SpeciesSynthetic
- Promoter CAG
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer pCAG-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
More information can be found in this additional reference: Yamagata M, Yan W, Sanes JR. A cell atlas of the chick retina based on single-cell transcriptomics. Elife. 2021 Jan 4;10:e63907. doi: 10.7554/eLife.63907. PMID: 33393903; PMCID: PMC7837701.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pXL-BacII-CAG-loxP-STOP-loxP-Venus was a gift from Joshua Sanes (Addgene plasmid # 170520 ; http://n2t.net/addgene:170520 ; RRID:Addgene_170520) -
For your References section:
CRISPR-mediated Labeling of Cells in Chick Embryos Based on Selectively Expressed Genes. Yamagata M, Sanes JR. Bio Protoc. 2021 Aug 5;11(15):e4105. doi: 10.21769/BioProtoc.4105. eCollection 2021 Aug 5. 10.21769/BioProtoc.4105 PubMed 34458399
