ZE18 pwnt8 ORF1/2 myc
(Plasmid
#17052)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 17052 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCS2+MT
- Backbone size w/o insert (bp) 4350
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namewnt8 ORF1/2
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SpeciesD. rerio (zebrafish)
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Entrez Genewnt8a (a.k.a. wnt-8, wnt8, wnt8.1, wu:fa20e02, wu:fe05d07)
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Tag
/ Fusion Protein
- 6xMyc (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
- 3′ cloning site ClaI (unknown if destroyed)
- 5′ sequencing primer SP6
- 3′ sequencing primer T3 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
wnt8 ORFs + IREs cloned into BamHI-ClaI sites of pCS2+MT. Adds 6 myc tags onto c-terminus of ORF2.
For Sense RNA: cut with Asp718 I and transcribe with SP6 polymerase.
Plasmid History:
Species of Sequence Origin: Zebrafish.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
ZE18 pwnt8 ORF1/2 myc was a gift from Randall Moon (Addgene plasmid # 17052 ; http://n2t.net/addgene:17052 ; RRID:Addgene_17052)