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pTR(EF)-tRNA(Q)
(Plasmid #170517)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 170517 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDONR221
  • Backbone size w/o insert (bp) 2352
  • Total vector size (bp) 2516
  • Vector type
    PCR template vector for amplifying gRNAcore(EF)-tRNA(Q) fragment; used together with pAC-U63-tgRNA-nlsBFP or pAC-U63-tgRNA-Gal80

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    gRNAcore(EF)-tRNA(Q)
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    164

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer M13 forward primer
  • 3′ sequencing primer M13 reverse primer
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For more information about the Han Lab Drosophila Transgenic Vectors, please visit: https://han.wicmb.cornell.edu/han-lab-drosophila-transgenic-vectors/

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTR(EF)-tRNA(Q) was a gift from Chun Han (Addgene plasmid # 170517 ; http://n2t.net/addgene:170517 ; RRID:Addgene_170517)

  • For your References section:

    Upgraded CRISPR/Cas9 tools for tissue-specific mutagenesis in Drosophila. Koreman GT, Xu Y, Hu Q, Zhang Z, Allen SE, Wolfner MF, Wang B, Han C. Proc Natl Acad Sci U S A. 2021 Apr 6;118(14). pii: 2014255118. doi: 10.1073/pnas.2014255118. 10.1073/pnas.2014255118 PubMed 33782117
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