pGC(2.1)-U6.3
(Plasmid #170516)

• Purpose: PCR template vector for amplifying gRNAcore(2.1)-pU6.3 promoter fragment; used together with pAC-CR7T-gRNA2.1-nlsBFP
• Depositing Lab: Chun Han
• Publication: Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118(14). pii: 2014255118. doi: 10.1073/pnas.2014255118.

Backbone

• Vector backbone: pDONR221
• Backbone size w/o insert (bp): 2351
• Total vector size (bp): 2869
• Vector type: PCR template vector for amplifying gRNAcore(2.1)-pU6.3 promoter fragment; used together with pAC-CR7T-gRNA2.1-nlsBFP.

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: gRNAcore(2.1)-pU6.3
• Species: D. melanogaster (fly)
• Insert Size (bp): 518

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: M13 forward primer
• 3′ sequencing primer: M13 reverse primer

Resource Information

• Supplemental Documents:
  • pGC(2.1)-U6.3.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For more information about the Han Lab Drosophila Transgenic Vectors, please visit: https://han.wicmb.cornell.edu/han-lab-drosophila-transgenic-vectors/