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Addgene

ZE16 pwnt8 ORF1-myc
(Plasmid #17050)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17050 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2+MT
  • Backbone size w/o insert (bp) 4350

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    wnt8
  • Species
    D. rerio (zebrafish)
  • Entrez Gene
    wnt8a (a.k.a. wnt-8, wnt8, wnt8.1, wu:fa20e02, wu:fe05d07)
  • Tag / Fusion Protein
    • 6xMyc (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site ClaI (unknown if destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

ORFs ligated into BamHI, ClaI cut pCS2+MT. Adds 6 myc tags onto carboxy terminus of each.
For Sense RNA: cut with Asp718 I and transcribe with SP6 polymerase.

Plasmid History:
Species of Sequence Origin: Zebrafish.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ZE16 pwnt8 ORF1-myc was a gift from Randall Moon (Addgene plasmid # 17050 ; http://n2t.net/addgene:17050 ; RRID:Addgene_17050)
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