SPELL-ITSN1(DH-PH) Component 2
(Plasmid
#170492)
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PurposeSPELL ITSN1(DH-PH) component 2. myc-FRB-ITSN(C-lobe). Split at loop 4 of DH domain.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 170492 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepTriEx
- Backbone size w/o insert (bp) 5000
- Total vector size (bp) 5850
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SPELL-ITSN1(DH-PH) Component 2 was a gift from Klaus Hahn (Addgene plasmid # 170492 ; http://n2t.net/addgene:170492 ; RRID:Addgene_170492) -
For your References section:
Computational design of chemogenetic and optogenetic split proteins. Dagliyan O, Krokhotin A, Ozkan-Dagliyan I, Deiters A, Der CJ, Hahn KM, Dokholyan NV. Nat Commun. 2018 Oct 2;9(1):4042. doi: 10.1038/s41467-018-06531-4. 10.1038/s41467-018-06531-4 PubMed 30279442