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Addgene

SPELL-ITSN1(DH-PH) Component 1
(Plasmid #170491)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 170491 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTriEx
  • Backbone size w/o insert (bp) 5000
  • Total vector size (bp) 6528
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mCherry, ITSN(N-lobe), iFKBP
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1533

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The plasmid includes a 4 amino acid -GS linker (SGGS) downstream of mCherry that is not included in the original uploaded sequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SPELL-ITSN1(DH-PH) Component 1 was a gift from Klaus Hahn (Addgene plasmid # 170491 ; http://n2t.net/addgene:170491 ; RRID:Addgene_170491)

  • For your References section:

    Computational design of chemogenetic and optogenetic split proteins. Dagliyan O, Krokhotin A, Ozkan-Dagliyan I, Deiters A, Der CJ, Hahn KM, Dokholyan NV. Nat Commun. 2018 Oct 2;9(1):4042. doi: 10.1038/s41467-018-06531-4. 10.1038/s41467-018-06531-4 PubMed 30279442
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