ZE15 pCS2P+ wnt8 ORF2
(Plasmid
#17049)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 17049 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCS2P+ (modified pCS2+)
- Backbone size w/o insert (bp) 4100
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namewnt8
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SpeciesD. rerio (zebrafish)
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Entrez Genewnt8a (a.k.a. wnt-8, wnt8, wnt8.1, wu:fa20e02, wu:fe05d07)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer SP6
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
ORF2 amplified from pBand 3-5 ligated into BamHI-StuI cut CS2P+.
For Sense RNA: cut with Asp718 I and transcribe with SP6 polymerase.
Plasmid History:
Species of Sequence Origin: Zebrafish.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
ZE15 pCS2P+ wnt8 ORF2 was a gift from Randall Moon (Addgene plasmid # 17049 ; http://n2t.net/addgene:17049 ; RRID:Addgene_17049)