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SPELL-pBabe-Vav2(DPZ) (Component 2-T2AP2A-Component 1)
(Plasmid #170488)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 170488 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBabe-tetoff-puro
  • Backbone size w/o insert (bp) 6000
  • Total vector size (bp) 8157
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FRB, Vav2(C-lobe), iFKBP, Vav2(N-lobe), mCherry
  • Species
    M. musculus (mouse)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The plasmid contains the Component2 - T2A P2A - Component1 fragment insert in a different backbone. Please refer to Addgene NGS result for full backbone sequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SPELL-pBabe-Vav2(DPZ) (Component 2-T2AP2A-Component 1) was a gift from Klaus Hahn (Addgene plasmid # 170488 ; http://n2t.net/addgene:170488 ; RRID:Addgene_170488)

  • For your References section:

    Computational design of chemogenetic and optogenetic split proteins. Dagliyan O, Krokhotin A, Ozkan-Dagliyan I, Deiters A, Der CJ, Hahn KM, Dokholyan NV. Nat Commun. 2018 Oct 2;9(1):4042. doi: 10.1038/s41467-018-06531-4. 10.1038/s41467-018-06531-4 PubMed 30279442
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