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Addgene

pRS_1N_400
(Plasmid #170476)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 170476 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBR322
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Tetracycline, 100 & 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    T7A1, O1, O-, Lambda t1 in lacZ fragment
  • Species
    Synthetic
  • Promoter T7A1

Cloning Information

  • Cloning method Unknown

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

LacI operators separated by 400 base pairs.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRS_1N_400 was a gift from Laura Finzi (Addgene plasmid # 170476 ; http://n2t.net/addgene:170476 ; RRID:Addgene_170476)

  • For your References section:

    Positive supercoiling favors transcription elongation through lac repressor-mediated DNA loops. Xu W, Yan Y, Artsimovitch I, Dunlap D, Finzi L. Nucleic Acids Res. 2022 Mar 21;50(5):2826-2835. doi: 10.1093/nar/gkac093. 10.1093/nar/gkac093 PubMed 35188572
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