pUCDMp10
(Plasmid
#170461)
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PurposeEarlyBac insect cell expression system
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 170461 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUCDM
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Pir1
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameNew vector
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
For a more detailed description, go to:
Furukawa, H, Simorowski, N, Michalski, K (January 2021) Effective production of oligomeric membrane proteins by EarlyBac-insect cell system. In: Methods in Enzymology. Elsevier.
DOI: 10.1016/bs.mie.2020.12.019
For online protocol, go to: http://furukawalab.labsites.cshl.edu/protocols/earlybac/
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUCDMp10 was a gift from Hiro Furukawa (Addgene plasmid # 170461 ; http://n2t.net/addgene:170461 ; RRID:Addgene_170461) -
For your References section:
Structural Basis of Functional Transitions in Mammalian NMDA Receptors. Chou TH, Tajima N, Romero-Hernandez A, Furukawa H. Cell. 2020 Jul 23;182(2):357-371.e13. doi: 10.1016/j.cell.2020.05.052. Epub 2020 Jun 30. 10.1016/j.cell.2020.05.052 PubMed 32610085