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Addgene

pFp10
(Plasmid #170460)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 170460 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFastBac
  • Backbone manufacturer
    Invitrogen
  • Total vector size (bp) 7229
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    none
  • Promoter dHsp70

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site multiple cloning site (unknown if destroyed)
  • 3′ cloning site multiple cloning site (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer n.a.
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For a more detailed description, go to:

Furukawa, H, Simorowski, N, Michalski, K (January 2021) Effective production of oligomeric membrane proteins by EarlyBac-insect cell system. In: Methods in Enzymology. Elsevier.
DOI: 10.1016/bs.mie.2020.12.019

For online protocol, go to: http://furukawalab.labsites.cshl.edu/protocols/earlybac/

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFp10 was a gift from Hiro Furukawa (Addgene plasmid # 170460 ; http://n2t.net/addgene:170460 ; RRID:Addgene_170460)

  • For your References section:

    Structural Basis of Functional Transitions in Mammalian NMDA Receptors. Chou TH, Tajima N, Romero-Hernandez A, Furukawa H. Cell. 2020 Jul 23;182(2):357-371.e13. doi: 10.1016/j.cell.2020.05.052. Epub 2020 Jun 30. 10.1016/j.cell.2020.05.052 PubMed 32610085
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