LentiU6-LacZ-GFP-Puro (BB)
(Plasmid #170459)

• Purpose: For cloning gRNA block
• Depositing Lab: Yonglun Luo
• Publication: Xiang et al Nat Commun. 2021 May 28;12(1):3238. doi: 10.1038/s41467-021-23576-0.

Backbone

• Vector backbone: 3rd Generation lentiviral vector
• Backbone size w/o insert (bp): 7466
• Total vector size (bp): 9882
• Modifications to backbone: no
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin ; EGFP

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: hU6-BB-LacZ and EF1A-EGFP-2A-Puro
• Species: Synthetic
• Promoter: hU6 for gRNA and EF1A for EGFP-2A-Puro

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: PacI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CAGGGACAGCAGAGATCCAGTTTGG
• 3′ sequencing primer: TGTTTCAGCAGAGAGAAGTTTGTTG

Resource Information

• Supplemental Documents:
  • LentiU6-LacZ-GFP-Puro (BB).gbk
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry