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Addgene

pSynapsin_NLS_GFP_P2A_His6_NR1.
(Plasmid #170457)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 170457 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFUGW
  • Backbone size w/o insert (bp) 9370
  • Total vector size (bp) 12124
  • Modifications to backbone
    UBC promoter replaced with neuron specific synapsin promoter. Introduced NLS-GFP for checking transfection efficiency.
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    NR1
  • Alt name
    NMDA receptor subunit 1
  • Species
    R. norvegicus (rat)
  • Entrez Gene
    Grin1 (a.k.a. GluN1, NMDAR1, NR1)
  • Promoter Synapsin
  • Tag / Fusion Protein
    • Hexa-histidin (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSynapsin_NLS_GFP_P2A_His6_NR1. was a gift from Shigeki Watanabe (Addgene plasmid # 170457 ; http://n2t.net/addgene:170457 ; RRID:Addgene_170457)

  • For your References section:

    Asynchronous release sites align with NMDA receptors in mouse hippocampal synapses. Li S, Raychaudhuri S, Lee SA, Brockmann MM, Wang J, Kusick G, Prater C, Syed S, Falahati H, Ramos R, Bartol TM, Hosy E, Watanabe S. Nat Commun. 2021 Jan 29;12(1):677. doi: 10.1038/s41467-021-21004-x. 10.1038/s41467-021-21004-x PubMed 33514725

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