pCDNA3.3_CoV2_B.1.1.7
(Plasmid #170451)

• Purpose: Express SARS-CoV-2 B.1.1.7 (UK strain / alpha strain) spike protein with an 18aa deletion on the C-terminal tail. Used for SARS2 (UK strain) pseudotyped virus production.
• Depositing Lab: David Nemazee
• Publication: Yuan et al Science. 2021 Aug 13;373(6556):818-823. doi: 10.1126/science.abh1139. Epub 2021 May 20.

Backbone

• Vector backbone: pcDNA3.3
• Backbone size w/o insert (bp): 5500
• Total vector size (bp): 9200
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SARS-CoV-2 501V2 spike D18
• Species: SARS-CoV-2, UK strain (B.1.1.7)
• Insert Size (bp): 3800
• Mutation: HY69-70del, Y144del, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H; 18aa deletion in c-terminal tail
• GenBank ID:
• Entrez Gene: S (a.k.a. GU280_gp02, spike glycoprotein)
• Promoter: CMV
• Tag / Fusion Protein:
  • NA

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: TK-pA-R

Resource Information

• Supplemental Documents:
  • SARS-CoV-1 pseudotyped virus (PSV) production protocol
• Articles Citing this Plasmid:
  • 13 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2021.02.16.430500v1 for bioRxiv preprint.