ZE4 Wnt-8b
(Plasmid
#17045)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 17045 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneT7Ts
- Backbone size w/o insert (bp) 3000
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Vector typeZebrafish Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namewnt-8b
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SpeciesD. rerio (zebrafish)
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Insert Size (bp)1100
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Entrez Genewnt8b (a.k.a. wnt-8b)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Insert: wnt-8b.
Insert size: ~1.1 Kb.
Transcription: linearize at Xba, transcribe with T7 RNA polymerase.
Plasmid History:
Constructed by Greg Kelly.
Species of Sequence Origin: Zebrafish.
Notes for Use:
Very little 5' or 3' UTR from wnt-8b clone.
*Use Hind III to linearize and SP6 RNA polymerase for in situ probes.
Citation: Kelly et al., Development 121, 1787 (1995)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
ZE4 Wnt-8b was a gift from Randall Moon (Addgene plasmid # 17045 ; http://n2t.net/addgene:17045 ; RRID:Addgene_17045) -
For your References section:
Zebrafish wnt8 and wnt8b share a common activity but are involved in distinct developmental pathways. Kelly GM, Greenstein P, Erezyilmaz DF, Moon RT. Development. 1995 Jun. 121(6):1787-99. PubMed 7600994
Map uploaded by the depositor.
