pCDH-EF1a-mCherry-EGFP-LC3B
(Plasmid #170446)

• Purpose: Lentiviral vector expressing tandem mCherry-EGFP-LC3B in mamalian cell. To visualize free autophagosome and autophagosomes that have fused with the lysosome.
• Depositing Lab: Sang-Hun Lee
• Publication: Wulansari et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabb1540. doi: 10.1126/sciadv.abb1540. Print 2021 Feb.

Backbone

• Vector backbone: pCDH-EF1a-T2A-copGFP
• Backbone manufacturer: System Biosciences
• Backbone size w/o insert (bp): 6607
• Total vector size (bp): 8533
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mCherry-GFP-LC3
• Alt name: MAP1LC3B
• Species: H. sapiens (human)
• Insert Size (bp): 1926
• GenBank ID: NM_022818.5
• Entrez Gene: MAP1LC3B (a.k.a. ATG8F, LC3B, MAP1A/1BLC3, MAP1LC3B-a)
• Promoter: EF1a

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: GGATCTCGACGGTATCGG

Resource Information

• Supplemental Documents:
  • EF1a-mCherry-EGFP-LC3B.ape.gbk
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry