pCDH-EF1a-hLMX1A-T2A-copGFP
(Plasmid #170444)

• Purpose: Lentiviral vector expressing human LMX1A (with silent mutation) with copGFP reporter gene, under control of EF1alpha promoter.
• Depositing Lab: Sang-Hun Lee
• Publication: Wulansari et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabb1540. doi: 10.1126/sciadv.abb1540. Print 2021 Feb.

Backbone

• Vector backbone: pCDH-EF1a-T2A-copGFP
• Backbone manufacturer: System Biosciences
• Backbone size w/o insert (bp): 7424
• Total vector size (bp): 8570
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: human LMX1A
• Alt name: LIM Homeobox Transcription Factor 1 Alpha
• Alt name: LMX1.1
• Insert Size (bp): 1146
• GenBank ID: NM_177398.4
• Promoter: EF1a

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: GGATCTCGACGGTATCGG

Resource Information

• Supplemental Documents:
  • pCDH-EF1a-LMX1A-CopGFP.ape.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry