pCDH-EF1a-hDNAJC6-T2A-copGFP
(Plasmid #170443)

• Purpose: Lentiviral vector expressing human DNAJC6 with copGFP reporter gene, under control of EF1alpha promoter
• Depositing Lab: Sang-Hun Lee
• Publication: Wulansari et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabb1540. doi: 10.1126/sciadv.abb1540. Print 2021 Feb.

Backbone

• Vector backbone: pCDH-EF1a-T2A-copGFP
• Backbone manufacturer: System Biosciences
• Backbone size w/o insert (bp): 7430
• Total vector size (bp): 10340
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: human DNAJC6
• Alt name: Auxilin
• Alt name: DnaJ Heat Shock Protein Family (Hsp40) Member C6
• Alt name: PARK19
• Species: H. sapiens (human)
• Insert Size (bp): 2910
• GenBank ID: BC109279.2
• Entrez Gene: DNAJC6 (a.k.a. DJC6, PARK19)
• Promoter: EF1a

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: GGATCTCGACGGTATCGG

Resource Information

• Supplemental Documents:
  • pCDH-EF1a-DNAJC6-CopGFP.ape.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry