Skip to main content
Addgene

pET-DEST42-dre4
(Plasmid #170440)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 170440 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pET-DEST42
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 7440
  • Total vector size (bp) 9154
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    dre4
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    3366
  • Mutation
    Deleted amino acids 1-20
  • Entrez Gene
    dre4 (a.k.a. Dmel_CG1828, CG1828, DRE4, DRE4/dSPT16, Dmel\CG1828, Dre4, SPT16, Spt16, dSPT16, dSpt16, dspt16, l(3)62Bg, l(3)dre4, spt16)
  • Promoter T7
  • Tags / Fusion Proteins
    • 6xHis (C terminal on backbone)
    • V5 (C terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The expressed his-tagged protein, pET-DEST42-dre4 (deleted amino acids 1-20) was efficiently bound to CNBR beads during the affinity purification of the antibodies.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-DEST42-dre4 was a gift from Joan Conaway (Addgene plasmid # 170440 ; http://n2t.net/addgene:170440 ; RRID:Addgene_170440)

  • For your References section:

    A Role for FACT in RNA Polymerase II Promoter-Proximal Pausing. Tettey TT, Gao X, Shao W, Li H, Story BA, Chitsazan AD, Glaser RL, Goode ZH, Seidel CW, Conaway RC, Zeitlinger J, Blanchette M, Conaway JW. Cell Rep. 2019 Jun 25;27(13):3770-3779.e7. doi: 10.1016/j.celrep.2019.05.099. 10.1016/j.celrep.2019.05.099 PubMed 31242411
Related items: