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pET-DEST42 -SSRP1
(Plasmid #170439)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 170439 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-DEST42
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 7440
  • Total vector size (bp) 7957
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    ssrp1
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    2169
  • Entrez Gene
    Ssrp (a.k.a. Dmel_CG4817, CG4817, Cf5, Dmel\CG4817, DssRP, SSRP, SSRP1, dSSRP, dSSRP1, dSsrp, ssrp)
  • Promoter T7
  • Tags / Fusion Proteins
    • 6xHis (C terminal on backbone)
    • V5 (C terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-DEST42 -SSRP1 was a gift from Joan Conaway (Addgene plasmid # 170439 ; http://n2t.net/addgene:170439 ; RRID:Addgene_170439)

  • For your References section:

    A Role for FACT in RNA Polymerase II Promoter-Proximal Pausing. Tettey TT, Gao X, Shao W, Li H, Story BA, Chitsazan AD, Glaser RL, Goode ZH, Seidel CW, Conaway RC, Zeitlinger J, Blanchette M, Conaway JW. Cell Rep. 2019 Jun 25;27(13):3770-3779.e7. doi: 10.1016/j.celrep.2019.05.099. 10.1016/j.celrep.2019.05.099 PubMed 31242411
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