ZE2 Wnt-4
(Plasmid #17043)

• Depositing Lab: Randall Moon
• Publication: Ungar et al Mech Dev. 1995 Aug;52(2-3):153-64. doi: 10.1016/0925-4773(95)00386-f.

Backbone

• Vector backbone: pT7TS
• Backbone size w/o insert (bp): 3000

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: wnt-4 cDNA
• Alt name: wnt4
• Species: D. rerio (zebrafish)
• Insert Size (bp): 1300
• Entrez Gene: wnt4a (a.k.a. wnt-4, wnt4, zgc:136737)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRV (not destroyed)
• 3′ cloning site: SpeI (not destroyed)
• 5′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Insert: wnt-4 (zebrafish) cDNA.
Insert size: 1.3 Kb.
To make an in situ probe, linearize with EcoRV and transcribe with SP6.
To make sense RNA, linearize with Xba1 and transcribe with T7.

Plasmid History:
Constructed by Anne Ungar on date of 6/8/93.
Species of Sequence Origin: Zebrafish.

Notes for Use:
Note presence of R1, Sma, BamH1 sites brought in from pBSSK during construction. The 1274 nucleotide Nsi1 fragment containing the complete wnt-4 coding region was ligated into the Pst1 site of pBSSK (zf4 in pBSSK clone 5). The 5' end is near the Kpn1 site. A 1.3 Kb fragment was cut out with EcoRV and Spe1, isolated and ligated into EcoRV/Spe1 cut pT7Ts.

Citation: Ungar et al., Mechanisms of Development 52 (1995)