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Addgene

pBEL2252
(Plasmid #170404)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 170404 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBAD-His
  • Backbone manufacturer
    Thermo Fisher
  • Backbone size w/o insert (bp) 4571
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LetAB
  • Alt name
    yebST
  • Species
    E. coli
  • Mutation
    LetA-LetB(Δ700-716, replace with 455-479)
  • Entrez Gene
    yebT (a.k.a. b1834, ECK1833)
  • Entrez Gene
    yebS (a.k.a. b1833, ECK1832)
  • Tag / Fusion Protein
    • 8xHis2xQH and TEV cleavage site on LetA (N terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBEL2252 was a gift from Gira Bhabha & Damian Ekiert (Addgene plasmid # 170404 ; http://n2t.net/addgene:170404 ; RRID:Addgene_170404)

  • For your References section:

    Role of Ring6 in the Function of the E. coli MCE Protein LetB. Vieni C, Coudray N, Isom GL, Bhabha G, Ekiert DC. J Mol Biol. 2022 Jan 22;434(7):167463. doi: 10.1016/j.jmb.2022.167463. 10.1016/j.jmb.2022.167463 PubMed 35077766
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