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Addgene

pBEL2116
(Plasmid #170393)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 170393 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pET17b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 3174
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LetAB
  • Alt name
    yebST
  • Species
    E. coli
  • Mutation
    LetA-LetB(Δ48-159)
  • Entrez Gene
    yebT (a.k.a. b1834, ECK1833)
  • Entrez Gene
    yebS (a.k.a. b1833, ECK1832)
  • Promoter T7

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBEL2116 was a gift from Gira Bhabha & Damian Ekiert (Addgene plasmid # 170393 ; http://n2t.net/addgene:170393 ; RRID:Addgene_170393)
  • For your References section:

    Role of Ring6 in the Function of the E. coli MCE Protein LetB. Vieni C, Coudray N, Isom GL, Bhabha G, Ekiert DC. J Mol Biol. 2022 Jan 22;434(7):167463. doi: 10.1016/j.jmb.2022.167463. 10.1016/j.jmb.2022.167463 PubMed 35077766