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Addgene

pAAV.U6gT28.4.hSyn.flex.H2B.RFP
(Plasmid #170386)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 170386 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cre inducible H2B RFP
  • Alt name
    Cre inducible H2B-mCherry
  • gRNA/shRNA sequence
    GGTCCTTGTGAGCGTTGAGC
  • Promoter human Synapsin

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer AGTCGTGTCGTGCCTGAGA
  • 3′ sequencing primer catagttaagaataccag
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV.U6gT28.4.hSyn.flex.H2B.RFP was a gift from Johan Jakobsson (Addgene plasmid # 170386 ; http://n2t.net/addgene:170386 ; RRID:Addgene_170386)

  • For your References section:

    Activation of endogenous retroviruses during brain development causes an inflammatory response. Jonsson ME, Garza R, Sharma Y, Petri R, Sodersten E, Johansson JG, Johansson PA, Atacho DA, Pircs K, Madsen S, Yudovich D, Ramakrishnan R, Holmberg J, Larsson J, Jern P, Jakobsson J. EMBO J. 2021 Mar 1:e106423. doi: 10.15252/embj.2020106423. 10.15252/embj.2020106423 PubMed 33644903
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