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pLV.U6.gLacZ.Cas9-T2A-GFP
(Plasmid #170361)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 170361 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLV
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cas9-T2A-GFP
  • gRNA/shRNA sequence
    TGCGAATACGCCCACGCGAT
  • Species
    H. sapiens (human)
  • Promoter U6

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV.U6.gLacZ.Cas9-T2A-GFP was a gift from Johan Jakobsson (Addgene plasmid # 170361 ; http://n2t.net/addgene:170361 ; RRID:Addgene_170361)

  • For your References section:

    Activation of neuronal genes via LINE-1 elements upon global DNA demethylation in human neural progenitors. Jonsson ME, Ludvik Brattas P, Gustafsson C, Petri R, Yudovich D, Pircs K, Verschuere S, Madsen S, Hansson J, Larsson J, Mansson R, Meissner A, Jakobsson J. Nat Commun. 2019 Jul 18;10(1):3182. doi: 10.1038/s41467-019-11150-8. 10.1038/s41467-019-11150-8 PubMed 31320637
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