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Addgene

PIP2-CFP(ST) sensor
(Plasmid #170355)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 170355 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CFP(ST)-PH
  • Species
    H. sapiens (human)
  • Entrez Gene
    PLCD1 (a.k.a. NDNC3, PLC-III)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PIP2-CFP(ST) sensor was a gift from Kees Jalink (Addgene plasmid # 170355 ; http://n2t.net/addgene:170355 ; RRID:Addgene_170355)

  • For your References section:

    Monitoring agonist-induced phospholipase C activation in live cells by fluorescence resonance energy transfer. van der Wal J, Habets R, Várnai P, Balla T, Jalink K. J Biol Chem. 2001 May 4. 276(18):15337-44. 10.1074/jbc.M007194200 PubMed 11152673
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