Epac-S-H81
(Plasmid #170339)

• Purpose: GFPDel(UST)_EPACdDEPCD_mRFP biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
• Depositing Lab: Kees Jalink
• Publication: van der Krogt et al PLoS ONE. 2008 . 3(4):e1916.

Backbone

• Vector backbone: pcDNA3
• Backbone size w/o insert (bp): 5500
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GFPDel(UST)_EPACdDEPCD_mRFP
• Species: H. sapiens (human)
• Mutation: Deletion of DEP domain, Catalytically dead T781A & F782A
• Entrez Gene: RAPGEF3 (a.k.a. CAMP-GEFI, EPAC, EPAC1, HSU79275, bcm910)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry