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Addgene

Epac-S-H74
(Plasmid #170338)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 170338 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mTDel_EPACdDEPCD_cp173Ven(ST)_Ven(ST)
  • Species
    H. sapiens (human)
  • Mutation
    Deletion of DEP domain, Catalytically dead T781A & F782A
  • Entrez Gene
    RAPGEF3 (a.k.a. CAMP-GEFI, EPAC, EPAC1, HSU79275, bcm910)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Epac-S-H74 was a gift from Kees Jalink (Addgene plasmid # 170338 ; http://n2t.net/addgene:170338 ; RRID:Addgene_170338)

  • For your References section:

    A mTurquoise-based cAMP sensor for both FLIM and ratiometric read-out has improved dynamic range. Klarenbeek JB, Goedhart J, Hink MA, Gadella TW, Jalink K. PLoS One. 2011 Apr 29;6(4):e19170. doi: 10.1371/journal.pone.0019170. 10.1371/journal.pone.0019170 PubMed 21559477
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