pKm-HCBE
(Plasmid #170333)

• Purpose: a modularized single-plasmid cytidine base editing (C-to-T) tool for Sinorhizobium meliloti
• Depositing Lab: Deqiang Duanmu
• Publication: Wang et al Front Microbiol. 2021 Jun 18;12:686008. doi: 10.3389/fmicb.2021.686008. eCollection 2021.

Backbone

• Vector backbone: pKm
• Vector type: CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Kanamycin, 25 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Rhizobium codon-optimized CBEs
• Species: Synthetic
• Mutation: Cas9n-D10A

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)
• 3′ cloning site: Unknown (unknown if destroyed)

Resource Information

• Supplemental Documents:
  • pKm-HCBE.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry