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Addgene

pKm-HCBE
(Plasmid #170333)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 170333 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pKm
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Kanamycin, 25 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Rhizobium codon-optimized CBEs
  • Species
    Synthetic
  • Mutation
    Cas9n-D10A

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKm-HCBE was a gift from Deqiang Duanmu (Addgene plasmid # 170333 ; http://n2t.net/addgene:170333 ; RRID:Addgene_170333)

  • For your References section:

    Highly Efficient CRISPR-Mediated Base Editing in Sinorhizobium meliloti. Wang L, Xiao Y, Wei X, Pan J, Duanmu D. Front Microbiol. 2021 Jun 18;12:686008. doi: 10.3389/fmicb.2021.686008. eCollection 2021. 10.3389/fmicb.2021.686008 PubMed 34220774
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