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Addgene

pESUMO-hRORg1
(Plasmid #170330)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 170330 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pESUMO
  • Backbone manufacturer
    LifeSensor
  • Backbone size w/o insert (bp) 5476
  • Total vector size (bp) 7297
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    RORG isoform 1
  • Alt name
    RORgamma
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1821
  • Entrez Gene
    RORC (a.k.a. IMD42, NR1F3, RORG, RZR-GAMMA, RZRG, TOR)
  • Promoter T7
  • Tag / Fusion Protein
    • HisSUMO (N terminal on backbone)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer ACCACTCCTTTAAGAAGGC
  • 3′ sequencing primer GCTAGTTATTGCTCAGCGG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pESUMO-hRORg1 was a gift from Patrick Griffin (Addgene plasmid # 170330 ; http://n2t.net/addgene:170330 ; RRID:Addgene_170330)

  • For your References section:

    Conformational Changes of RORgamma During Response Element Recognition and Coregulator Engagement. Strutzenberg TS, Zhu Y, Novick SJ, Garcia-Ordonez RD, Doebelin C, He Y, Chang MR, Kamenecka TM, Edwards DP, Griffin PR. J Mol Biol. 2021 Sep 20;433(22):167258. doi: 10.1016/j.jmb.2021.167258. 10.1016/j.jmb.2021.167258 PubMed 34547329
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